Technology is having an impact on the the rapidly growing cannabis industry. CNET executive editor Roger Cheng joins CBSN to discuss developments, from …
How ridiculous. Weed is weed is weed. Always someone trying to make more money out of what should be affordable to those who enjoy drugging. You can still say no to drugs.
In some ways the cousin of cannabis, the hemp plant provides superior effects, and this is why believe it or not, Endoca provides suppositories. This is also why the World Health Organization wanted CBD declassed. BMC Complement Altern Med. 2016 Sep 1;16(1):335. doi: 10.1186/s12906-016-1280-0. Cannabidiol rather than Cannabis sativa extracts inhibit cell growth and induce apoptosis in cervical cancer cells. Lukhele ST1, Motadi LR2. Author information Abstract BACKGROUND: Cervical cancer remains a global health related issue among females of Sub-Saharan Africa, with over half a million new cases reported each year. Different therapeutic regimens have been suggested in various regions of Africa, however, over a quarter of a million women die of cervical cancer, annually. This makes it the most lethal cancer amongst black women and calls for urgent therapeutic strategies. In this study we compare the anti-proliferative effects of crude extract of Cannabis sativa and its main compound cannabidiol on different cervical cancer cell lines.
METHODS: To achieve our aim, phytochemical screening, MTT assay, cell growth analysis, flow cytometry, morphology analysis, Western blot, caspase 3/7 assay, and ATP measurement assay were conducted.
RESULTS: Results obtained indicate that both cannabidiol and Cannabis sativa extracts were able to halt cell proliferation in all cell lines at varying concentrations. They further revealed that apoptosis was induced by cannabidiol as shown by increased subG0/G1 and apoptosis through annexin V. Apoptosis was confirmed by overexpression of p53, caspase 3 and bax. Apoptosis induction was further confirmed by morphological changes, an increase in Caspase 3/7 and a decrease in the ATP levels.
CONCLUSIONS: In conclusion, these data suggest that cannabidiol rather than Cannabis sativa crude extracts prevent cell growth and induce cell death in cervical cancer cell lines.
a scale that tells you how much you are consuming, really? You can't tell that by how much is left in your stash? WTF
Go green!!! 🙂
Many people believe that teen marijuana use is not harmful. Nothing could be further from the truth.
How ridiculous. Weed is weed is weed. Always someone trying to make more money out of what should be affordable to those who enjoy drugging. You can still say no to drugs.
Elitist stoner 😂
The weed is outrageously expensive. And not that good.
Banana cream? Yes please.
So did you all by a Mega Millions Ticket today ? If you win you can 🚬 and 🍺 all you want !!
Ugh… just say no to drugs
How much is a 1/4 once going for nowadays? I paid $20 back in the mid 80s
Just take it off the list as a Class A drug. Deregulate it.
Science has been there since the 80s but the gov blocked it so no one knows wtf is up
In some ways the cousin of cannabis, the hemp plant provides superior effects, and this is why believe it or not, Endoca provides suppositories. This is also why the World Health Organization wanted CBD declassed.
BMC Complement Altern Med. 2016 Sep 1;16(1):335. doi: 10.1186/s12906-016-1280-0.
Cannabidiol rather than Cannabis sativa extracts inhibit cell growth and induce apoptosis in cervical cancer cells.
Lukhele ST1, Motadi LR2.
Author information
Abstract
BACKGROUND:
Cervical cancer remains a global health related issue among females of Sub-Saharan Africa, with over half a million new cases reported each year. Different therapeutic regimens have been suggested in various regions of Africa, however, over a quarter of a million women die of cervical cancer, annually. This makes it the most lethal cancer amongst black women and calls for urgent therapeutic strategies. In this study we compare the anti-proliferative effects of crude extract of Cannabis sativa and its main compound cannabidiol on different cervical cancer cell lines.
METHODS:
To achieve our aim, phytochemical screening, MTT assay, cell growth analysis, flow cytometry, morphology analysis, Western blot, caspase 3/7 assay, and ATP measurement assay were conducted.
RESULTS:
Results obtained indicate that both cannabidiol and Cannabis sativa extracts were able to halt cell proliferation in all cell lines at varying concentrations. They further revealed that apoptosis was induced by cannabidiol as shown by increased subG0/G1 and apoptosis through annexin V. Apoptosis was confirmed by overexpression of p53, caspase 3 and bax. Apoptosis induction was further confirmed by morphological changes, an increase in Caspase 3/7 and a decrease in the ATP levels.
CONCLUSIONS:
In conclusion, these data suggest that cannabidiol rather than Cannabis sativa crude extracts prevent cell growth and induce cell death in cervical cancer cell lines.
Everytime I think of, see, smell weed, I get happy, happy, happy 😁
I want to Work there pls !!!
By the way ! First !